Objective：To investigate the prevalence of osteoporosis and the changes of bone metabolic markers in patients with connective tissue disease（CTD）by measuring bone mineral density and bone metabolism index，and evaluate the effect of YunKe on the patients with rheumatoid arthritis（RA）. Methods：In the study，280 patients with connective tissue disease in the Affiliated Hospital of Jiangsu University from February 2016 to February 2017 were recruited. Among them，there were 75 cases of systemic lupus erythematosus（SLE），45 cases of primary Sjogren syndrome（pSS）and 160 cases of RA. Bone mineral density（BMD）of lumbar spine L1-L4 and the proximal femur were measured using dual energy X-ray absorptiometry（DXA）in all patients. The bone metabolic markers，serum osteocalcin（OC），N-terminal propeptide of type Ⅰ procollagen（PINP）and C-terminal cross-linking telopeptide of type I collagen（CTX）were also detected by electro chemiluminescence immunoassay. The bone metabolism index OC，PINP and CTX were analyzed respectively with age，duration，body mass index（BMI），C-reactive protein（CRP）and erythrocyte deposition rate（ESR），complement C3，rheumatoid factor（RF），bone density，and hormone usage. Seventy RA patients were treated with YunKe for 4 months and the effect of YunKe on the bone metabolic markers was evaluated. Results：A total of 280 patients had a mean age of（53.96 ± 14.10）years，mean duration of（94.88 ± 108.40）months and mean BMI of（21.84 ± 2.27）kg/m2，in which 27.7% patients showed osteopenia and 21.5% patients showed osteoporosis. Compared with the SLE patients，OC，PINP and CTX levels gradually increased in pSS and RA patients. OC，PINP and CTX levels in pSS and RA patients also gradually increased in the osteopenia group and the osteoporosis group. There were significant differences between normal bone mass group and osteoporosis group（P < 0.05）. OC，PINP and CTX levels in pSS were positively correlated with serum RF（OC：r1=0.570，P=0.002；PINP：r2=0.752，P=0.000；CTX：r3=0.660，P=0.000）；OC and PINP levels in SLE were negatively correlated with BMD of hip（OC：r1=-0.382，P=0.028；PINP：r2=-0.527，P=0.002）；OC and CTX levels in pSS were negatively correlated with BMD of hip（OC：r1=-0.471，P=0.013；CTX：r3=-0.422，P=0.028）. The level of OC was significantly increased（P < 0.05）and the level of ESR was significantly decreased（P < 0.05）after treated with YunKe. The differences of OC and ESR were statistically significant，while the levels of PINP，CTX，CRP and BMD were no significant differences between before and after 4 months of the treatment. Conclusion：Osteoporosis in connective tissue disease patients is common and there are different degrees of bone metabolism changes. Bone metabolic markers can probably predict bone loss in the early stage. YunKe has good effect on bone metabolism for RA.