Objective：To compare the difference of Hematoxylin eosin（HE） staining，P16 and Ki67 protein expression，as well as hTERC gene hybridization effect between environmental transparent dewaxing agent Van-clear and traditional transparent dewaxing agent xylene in different cervical lesions，and to explore the practical value of Van-clear. Methods：A total of 42 cervicitis specimens，53 cervical intraepithelial neoplasia（CIN）Ⅰspecimens，61 CIN Ⅱ specimens，56 CIN Ⅲ specimens，and 48 cervical cancer specimens were collected from Shantou Central Hospital from January 2016 to July 2018. In group A，260 paraffin sections were made from xylene，while in group B，260 paraffin sections were made from Van-clear. HE staining，immunohistochemistry（IHC）staining，and fluorescence in situ hybridization（FISH）of hTERC gene were used to detect the difference of HE staining excellence rate，P16 and Ki67 protein expression，and hTERC gene positive rate between the two groups. Results：①Sections in group A and group B for HE staining and immunohistochemistry staining all met the quality control standard of industry. ②The excellent and good rates of HE staining in group A and group B were 98.08% and 99.23%，respectively. There was no significant difference between the two groups（χ2=1.30，P=0.25）. ③Comparison of positive rate of IHC staining showed there was no significant difference in the positive rate of P16 and Ki67 protein in different cervical lesions between groups A and gronp B（P＞0.05）.④Comparison of the positive rate of hTERC gene amplification showed there was no significant difference between group A and gronp B in different cervical lesions（P＞0.05），and no significant difference was observed between the two groups under fluorescence microscope. Conclusion：Van-clear，as a new environmental transparent dewaxing agent，has the same effect as traditional transparent dewaxing agent xylene in HE staining，P16 and Ki67 protein expression，and hTERC gene detection in different cervical lesions. It is also green and environment-friendly，we recommend that it be widely used in clinic.