Objective：This study aims to investigate the expression and significance of tumor suppressor SUFU（Suppressor of Fused）in ovarian cancer. Methods：We detected the expression of SUFU in human ovarian cancer and adjacent tissues by immunohistochemistry，and verified the results in large samples and at the cellular level. Then MTT，EdU，flow cytometry，Western blot，Transwell，cloning formation experiments were used to detect cell proliferation，apoptosis，epithelial-mesenchymal transition（EMT），migration and invasion after regulating the expression of SUFU in the human normal ovarian epithelial cell line IOSE80 and the ovarian cancer cell line SKOV3 respectively. Meanwhile，the protein and mRNA levels of target gene GLI1 in the Sonic Hedgehog（SHH）signaling pathway were detected by Western blot and quantitative real-time PCR（qPCR）. Results：In three ovarian cancer tissue samples，SUFU in cancer tissues was downregulated compared to adjacent tissues，and this result was also confirmed in large samples of the TCGA database. In addition，at the cellular level，SUFU was higher in the human normal ovarian epithelial cell line IOSE80 than in the ovarian cancer cell line SKOV3. Further overexpression of SUFU in SKOV3 cells can effectively inhibit proliferation，EMT，colony formation，migration and invasion，and promote apoptosis；on the contrary，downregulation of SUFU in IOSE80 cells can promote cell EMT，migration and invasion. At the same time，GLI1 mRNA and protein levels were significantly