Objective：This study aims to investigate the effects of ovarian tissue endogenous peptide 1 derived from AHNK2 protein（P1DA）on the proliferation，migration，invasion and apoptosis of ovarian cancer cell lines of SKOV3 and OVCAR3. Methods：SKOV3 and OVCAR3 cells in logarithmic growth phase were treated with P1DA. The cell activity was detected by CCK-8 assay to explore the effect of P1DA on the proliferation of SKOV3 and OVCAR3 cells. Transwell experiment was performed to detect the migration and invasion of SKOV3 and OVCAR3 cells treated with P1DA. Flow cytometry analysis was performed to analyze the apoptosis of SKOV3 and OVCAR3 cells treated with P1DA. And the effects of P1DA on apoptosis of ovarian cancer cells were analyzed. Results：The cell activity of SKOV3 and OVCAR3 cells treated with P1DA was significantly increased，the migrated and invaded cells in chamber were increased，and no difference was found in apoptosis of cells after treated with P1DA. Conclusion：Ovarian tissue endogenous peptide P1DA can promote the proliferation，migration and invasion of SKOV3 and OVCAR3，but has no effect on cell apoptosis.