Objective：This study aims to investigate the expression of miR-588 in breast cancer and its effects on proliferation，migration and invasion of breast cancer cells. Methods：The miR-588 was synthesized by chemical synthesis（miR-588 mimic），and the over expression rate of miR-588 was verified. MTT and clonogenic assay were used to detect the effect of miR-588 on the proliferation of breast cancer cells of MCF7 and MDA-MB-231. The effects of miR-588 on migration and invasion of MDA-MB-231 cells were detected by scratch healing test and Transwell invasion test. Results：MiR-588 expression in breast cancer tissues were lower than those in adjacent normal tissues and normal breast epithelial cells；Overexpression of miR-588 could inhibit proliferation of MCF7 and MDA-MB-231 cells；upregulation of miR-588 could significantly inhibit the migration and invasion of MDA-MB-231 cells. Conclusion：The expression of miR-588 is low in breast cancer. Overexpression of miR-588 can inhibit the proliferation and invasion of breast cancer cells.