Objective：To explore the sequence variation of the promoter region（-436~+267）on stimulator of interferon genes（STING）of children. Methods：The sequences of the promoter region of 48 children were amplified by PCR and then sequenced. The luciferase reporter plasmid pGL-3/STING（TT）and pGL-3/STING（CC）were constructed to transfect human embryonic kidney（HEK）-293T cells， and then the luciferase gene expression was detected and the relative luciferase activity unit（RLU）was calculated. Results：A new single nucleotide polymorphism（SNP）T/C（-401）in STING promoter region was identified. The frequencies of genotype TT，TC，and CC were 45.8%，37.5%，and 16.7% respectively. Restriction endonuclease analysis and DNA sequencing verified the successful construction of the plasmid pGL-3/STING（TT）and pGL-3/STING（CC）including the SNP. Compared with plasmid pGL-3/STING（TT），pGL-3/STING（CC）exhibited a weaker promoter activity. Conclusion：T/C（-401）in STING promoter region is a new SNP. The genotype of this new SNP may reduce the activity of STING promoter，and consequently affect the transcription and expression of STING gene，and affect the body’s susceptibility to disease.