Objective：This study aims to screen for asthma-related candidate genes and validate at the population and animal levels to explore the pathogenesis of asthma. Methods：The asthma-related sputum chip was searched from Gene Expression Omnibus（GEO），the differentially expressed genes were screened by GEO 2R，and the asthma-related genes were screened by reference to the whole gene association analysis（GWAS）. Asthmatic children and healthy children’s sputum specimens werecolle cted. Mouse asthma model construct and rat mast cell strain（RBL-2H3）asthma model were constructed，and real-time fluorescence quantitative polymerase chain reaction（RT-PCR）was used for differential expression verification. Results：Two sets of asthmatic gene chip data were included，and 190 differentially expressed genes with crossovers were selected. GWAS was used to finally screen out the asthma-related gene PDE4D. It was further verified that the expression of PDE4D was increased in the sputum samples of asthmatic children（P < 0.05），and decreased in the whole lung tissue of the asthma mouse model（P < 0.05），but up-regulated in the sensitized RBL-2H3 cells（P < 0.05）. Conclusion：PDE4D is differentially expressed in human，asthmatic mice and mast cells，which may serve as a research target for mast cells to participate in the development of asthma.