Single nucleotide polymorphism（SNP）refers to the DNA polymorphism caused by the variation of the single nucleotide in the level of genotype. SNP，as a kind of bialleic marker，is characterised by the high density，low mutation rate and uneven distribution. In the field of forensic medicine，SNP is applied for the inference of identity，lineage，ancestry and phenotype. However，the current first generation sequencing cannot analyze SNP effectively. Owning to the development of next generation sequencing（NGS），SNP processing speed improved greatiy. Therefore，the application of NGS to analyze SNP has great potential and broad prospects in the forensic medicine.