Objective：To idefine the regions of the human short isoform thymic stromal lymphopoietin（sfTSLP）promoter and to predict the potential transcription factor binding sites. Methods：Original human promoter fragments were isolated by PCR，using primers generated from genomic sequences of sfTSLP. Amplified fragments were then cloned into the pGL3-basic vector. Five promoter fragments with different length were obtained by walking deletion and cloned into pGL3-basic vector. The vector expression activities were determined by measuring luciferase activity in HEK-293 cells. Its potential transcriptional binding sites were predicted by bioinformatics methods. Results：A nested series of deletion constructs of the human sfTSLP gene promoter were generated and the core promoter region was determined to be located in the -200~+25 region at the 5′ side，which may contain the binding sites of SP1/SP3，SPI1/SPIB，TCF3，ETS1 and other transcription factors. Conclusion：It is the first study on the promoter fragmentsof human sfTSLP. The core promoter region was found and the possible transcription factor binding sites were also preliminarily identified，which can be the basis of further study.