Objective：To observe the effects of human adiponectin receptor（AdipoR1）gene overexpression and knockdown on PC9 cells proliferation and migration，and to clarify the regulatory effect of AdipoR1 on lung cancer. Methods：The expression levels of AdipoR1 in PC9 cells and control HBEC were deteceted by RT-PCR. The overexpression vector and shRNA interference vector of AdipoR1 were established by molecular clone method and transfected into PC9 cells，the expression levels of AdipoR1 mRNA and protein were detected by RT-PCR and Western blot. CCK8 method and scratch healing experiment were used to detect the effect of AdipoR1 agonist AdipoRon，AdipoR1 overexpression，and AdipoR1 knockdown on proliferation and migration ability of PC9 cells. Results：RT-PCR results showed that the expression of AdipoR1 was decreased in PC9 cells（P < 0.05）. RT-PCR and Western blot results showed that mRNA and protein expression levels of AdipoR1 were up-regulated in AdipoR1 overexpressed PC9 cells（P < 0.05），and down-regulated in AdipoR1 knockdown PC9 cells（P < 0.05）. CCK8 and scratch healing results showed that the proliferation and healing percentage of PC9 cells treated by AdipoRon for 24 or 48 h were significantly decreased when compared with the vehicle group（all P< 0.05）. The proliferation and healing percentage were also significantly lower in AdipoR1 overexpression PC9 cells and higher in AdipoR1 knockdown PC9 cells than theircontrols after cultured for 24 or 48 h（all P < 0.05）. Conclusion：AdipoR1 agonist AdipoRon，AdipoR1 overexpression can inhibit the proliferation and migration ability of lung cancer PC9 cells，while AdipoR1 knockdown can increase the proliferation and migration ability of lung cancer PC9 cells.