Objective：To observe the effects of endotoxin tolerance induced by Porphyromonas gingivalis（P.gingivalis）on the secretion of metalloproteinase（MMP）-1 and MMP-7 in peritoneal macrophages of mice and the wound-healing abilities of L929 cells. Methods：Endotoxin tolerance in murine peritoneal macrophages was triggered by repeated 1 μg/mL P.gingivalis lipopolysaccharide（LPS）stimulations，and 1 μg/mL Escherichia coli（E.coli） LPS was served as positive control. Supernatants from tolerized macrophages were collected and levels of MMP-1 and MMP-7 were determined by ELISA. In addition，L929 cells were stimulated by supernatants from tolerized macrophages and their wound-healing abilities were evaluated by repair areas after scratch. Results：Secretion of MMP-1 in macrophages decreased significantly after repeated stimulation by P.gingivalis LPS compared with that treated by single P.gingivalis LPS stimulation（P < 0.05），while there were no differences in MMP-7 levels between the two groups（P > 0.05）. Repaired areas after scratch（12 h，24 h）in L929 cells cultured with supernatants from P.gingivalis LPS-tolerized macrophages were bigger than those from P.gingivalis LPS non-tolerized macrophages（P < 0.05）. Conclusion：Endotoxin tolerance triggered by P.gingivalis LPS contributes to the decreased secretion of MMP-1 in murine peritoneal macrophages and the enhanced migration of L929 cells.