In developmental biology，chimera refers to the mixed state that cells or tissues with different genotypes contact and coexist in the same individual. If the cells come from different species，the individual is called interspecies-chimera，whereas the chimera composed of cells coming from the same species is called the intraspecies-chimera. In this paper，we review the origin of the interspecies-chimaera，and analyze the development progress of the interspecies-chimaeras from different types of stem cells and the key factors of their formation. The application of the interspecies-chimaera in research fields and their clinical development are envisaged.