Objective：This stady aims to observe the role of hepatocyte growth factor（HGF） derived from gastric cancer mesenchymal stem cells（GC-MSC）in the proliferation，migration and tumor formation of gastric cancer cells. Methods：The proliferation activity of gastric cancer cells was detected with CCK8 assay. The colony forming ability of gastric cancer cells was analyzed via colony formation assay. Transwell assay and cell wound scratch assay were used to measure the migration ability of gastric cancers. By methods of Western blot and flow cytometry，we detected the expression of programmed cell death-ligand 1（PD-L1）in gastric cancer cells or cancer tissues. BALB/c nu/nu subcutaneous tumor model was constructed to observe tumor formation in vivo. The expression levels of HGF in serum and culture media were determined by ELISA，and protein levels of HGF in tissues of gastric cancer patients and healthy people were detected by immunohistochemistry. Results：The content of HGF in culture medium of gastric cancer mesenchymal stem cells（GC-MSC-CM） was significantly higher than that in culture medium of bone marrow mesenchymal stem cell（BM-MSC-CM）（P < 0.05）. GC-MSC-CM treatment enhanced the proliferation，migration and colony formation of gastric cancer cells，and the promoting effect could be inhibited by HGF blockade. GC-MSC-CM could also promote the expression of PD-L1 in gastric cancer cell lines，and the proportion of PD-L1 positive cells decreased after HGF inhibition. In addition，exogenous HGF could also promote proliferation and PD-L1 expression of gastric cancer cells. The serum HGF levels in gastric cancer patients before therapy were significantly higher than those in healthy people（P < 0.05）. Conclusion：GC-MSC-CM-derived HGF plays an important role in promoting gastric cancer cells proliferation，migration，colony formation，PD-L1 expression and tumor formation，which may become apotential molecular target for gastric cancer prevention and treatment.