Objective：This stady aims to observe the effects of continuous exposure to static magnetic field（SMF）on the proliferation and differentiation of dental pulp stem cells（DPSCs）. Methods：The magnetic cell culture set was used with the flux intensity about （35 ± 5）mT. The normal cultured cells were used as control. The morphology of the cells was detected by scanning electron microscopy（SEM）. The cell proliferation was detected by CCK-8，and the alkaline phosphatase activity（ALP）was also measured. Alizarin red staining（ARS） was used to detect the mineral synthesis. Results：There were no significant differences in SEM images and cell proliferation between magnetic cultured cells and normal cultured cells，but the ALP activity of magnetic cultured cells was significantly increased（P < 0.05），and the mineralization formation of cells under magnetic culture was significantly more than that of normal cultured cells（P < 0.05）. Conclusion：Continuous exposure to SMF neither changed the cell morphology nor the cell proliferation，but significantly promoted the differentiation of DPSCs.