Objective：The aim of this study was to provide the reference for the diagnosis and differential diagnosis of related diseases，especially subacute thyroiditis by determining the general and regional distribution of the susceptible gene HLA-B*35 and its subtypes in Chinese mainland. Method：HLA high resolution typing was performed in 941 902 healthy adults in China. By classifing into 31 provinces，autonomous regions and municipalities，the overall and regional distribution characteristics of healthy adults and their HLA-B*35 genes and their subtypes in mainland China were determined. Results：The frequency of HLA-B *35 gene in healthy adult population in mainland China was higher in the northern population than that in the other regions. According to the classification of regions，the frequency of HLA-B *35 gene was the highest in Northwest China and was lower in South China. According to the calculation of provinces，municipalities and autonomous regions，the results showed that the gene frequency ranged from high to low in Inner Mongolia Autonomous Region（11.99%），Xinjiang Uygur Autonomous Region（11.76%），Ningxia Hui Autonomous Region（11.75%），Qinghai Province（11.61%）and Gansu Province（11.14%）. The lowest frequency was in Guangxi Zhuang Autonomous Region（3.79%）. The frequency of HLA-B* 3501 and HLA-B*3503 were the highest in Northwest China and the lowest in South China when classified by region. According to statistics of provinces and autonomous regions，the frequency of B*3501 is the highest in Inner Mongolia autonomous region and the lowest in Guangxi Zhuang autonomous region. Correspondingly，the frequency of B*3503 is the highest in Qinghai province and the lowest in Zhejiang province. Conclusion：The results showed that the distribution of HLA-B *35 in mainland China was high in the north and low in the south. Our study was conducted to explore the distribution of HLA-B*35 and its subtypes in different regions which is helpful for the diagnosis and differential diagnosis of related diseases.