Objective：This Study aims to study the effect of silencing general control nonderepressible 5（GCN5）or SRY related HMG box-9（SOX9）gene on TGF-β1 production in the renal tissue of Thy-1 nephritis（Thy-1N） rats. Methods：The plasmids of GCN5 and SOX9 shRNA were packaged with lentiviral（LV）to form LV-shGCN5 and LV-shSOX9. Then，the LV-shGCN5 and LV-shSOX9 vectors were separately perfused into rat kidneys by renal artery perfusion，and next，the rat Thy-1N model was replicated by injection of rabbit anti-rat-thymocyte serum（ATS）through rat tail vein. Thereafter，rat renal tissues mentioned-above were taken at 3 h after ATS injection，and mRNA and protein levels of GCN5，SOX9 and TGF-β1 were examined by RT-PCR and Western blot to verify the effects of GCN5 or SOX9 gene interference on TGF-β1 expression in the renal tissues of rats with Thy-1N. Results：LV-shGCN5 or LV-shSOX9 vectors could be perfused successfully into rat kidneys through renal artery，and LV-shGCN5 or LV-shSOX9 not only effectively silenced GCN5 or SOX9 gene，but also downregulated TGF-β1 synthesis in the renal tissue of Thy-1N rats. Conclusion：Knockdown of GCN5 or SOX9 gene can significantly inhibit renal TGF-β1 production of rats with Thy-1N.