Objective：This study aims to investigate the expression of LAPTM4B-35 protein in normal salivary gland and salivary adenoid cystic carcinoma，and to explore the correlation between LAPTM4B-35 protein expression and clinicopathological characteristics of tumor patients. Methods：The clinical and pathological data of 95 cases of adenoid cystic carcinoma and 20 cases of normal salivary gland were collected. By immunohistochemistry analysis，LAPTM4B-35 expression was evaluated in salivary adenoid cystic carcinoma tissues and normal salivary tissues. The correlation of LAPTM4B-35 expression with clinic-pathological parameters was assessed using Chi-square or Fisher’s exact test and Logistic regression analysis. Results：The expression level of LAPTM4B-35 was variant among different cell types of normal salivary glands. It was expressed fairly low in serous and mucous acini，weak in intercalated duct and excretory duct cells，moderate in secretory/striated ducts. In 48.00% of high grade salivary adenoid cystic carcinoma tested，LAPTM4B-35 was high expression，which was significantly higher than that in low grade salivary adenoid cystic carcinoma. The expression rate of LAPTM4B-35 were significantly associated with histological grades and clinical stage. Conclusion：LAPTM4B-35 protein was differentially expressed in normal salivary gland cells，and expression of LAPTM4B-35 protein was significantly related to the histological grade and clinical stage of adenoid cystic carcinoma.