After the completion of the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) isolation,culture and genome sequencing,the diagnosis of new coronavirus pneumonia mainly depends on the detection of the SARS-CoV-2 nucleic acid by RT-PCR. In addition,the new room temperature nucleic acid amplification technologies such as recombinase aided amplification(RAA)have been further applied in lab detection. Immunological diagnosis includes colloidal gold and IgM/IgG antibody detection by ELISA technology,which is used for screening and auxiliary diagnosis of the population. New diagnostic technologies based on CRISPR are also in the process of further improvement.
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ZHOU Tingting, ZHU Jin. Application and laboratory detection of severe acute respiratory syndrome coronavirus⁃2[J].,2020,(4):474-477.