Objective：To identify and analyze the elongation factor Tu GTP-binding domain-containing 2（EFTUD2）promoter region for the further study of the gene in transcriptional control and expression. Methods：The EFTUD2 gene structure and potential promoter regions were analyzed by bioinformatics methods，and based on which four EFTUD2 promoter sequences were predicted. Whole-genome synthesis technology and high-fidelity PCR amplification were used to obtain the target promoter sequence. The target band was cloned into the dual luciferase reporter vector psiCHECK-2 by BglⅡ and NheⅠ double digestion，and four different EFTUD2 gene promoter luciferase reporter recombinants covering about 2 kb upstream of the EFTUD2 gene transcription initiation site were obtained. Luciferase assay was used to detect promoter activity. Results：Luciferase reporter assays demonstrated that，compared with the control group，the relative luciferase activity of the EFTUD2-0.5 recombinant increased（P < 0.05），suggesting that the EFTUD2 core promoter may be located in a region of 500 bp near the transcription initiation site. Conclusion：It was identified that the 500 bp upstream of the EFTUD2 gene transcription initiation site has promoter activity，and it is speculated that its core promoter is located in this region.