Objective：To investigate the distribution of resistant determinants in drug-resistant Enterococcus faecalis（E. faecalis），and their relationships among strains. Methods：Twenty strains of E. faecalis were collected from Jianghan Oil Field General Hospital in Hubei from January 2015 to December 2018. Then，1 kind of resistance gene to penicillin， 4 kinds of resistance genes to aminoglycosides，6 kinds of resistance genes to macrolides，1 kind of resistance gene to streptogramin，2 kinds of resistance genes to tetracycline，3 kinds of resistance genes to vancomycin，2 kinds of resistance genes to quaternary ammonium disinfectants，2 kinds of genetic markers of mobile genetic elements were analyzed by PCR. At last，sample cluster analysis was performed（UPGMA method）. Results：At least 1 resistant determinants，and 7 at the most，were positive in 20 strains of drug-resistant E. faecalis. And 1 kind of resistance gene to penicillin，3 kinds of resistance genes to aminoglycosides，1 kind of resistance genes to macrolides，1 kind of resistance genes to tetracycline，2 kinds of resistance genes to vancomycin，1 kind of genetic markers of mobile genetic elements were positive. The analysis of phylogenetic relationship showed that 20 strains could be divided into A and B two groups. The B group can be divided into 2 subgroups：B-1 and B-2. There were 2 clones in the subgroup B-1（strain No. 4 -14 -17，and strain No. 8 -11）. As well as a clone in the subgroup B-2（strain No. 9-12）. Conclusion：Resistance gene to penicillin，aminoglycosides，macrolides，tetracycline，vancomycin，and genetic markers of mobile genetic elements play a key role in resistance to antimicrobial agents.