Objective：To investigate the expression of miR-552-3p in hepatocellular carcinoma（HCC）and its mechanism of promoting cell proliferation，migration and invasion. Methods：qRT-PCR was used to detect the expression of miR-552-3p in HCC cell lines and normal human liver cell lines. The microarray data of HCC samples were downloaded from TCGA database，and the expressions of miR-552-3p in HCC tumor tissues and its adjacent tissues were analyzed to show whether they are different or not；miR-552-3p mimics and miR-552-3p inhibitor transfected HCC cells，and luciferase assays verified whether miR-552-3p targets DACH1，while CCK8 and Transwell assays detected the effect of miR-552-3p and its interaction with DACH1 on HCC cell proliferation，migration，and invasion. Results：The analysis of HCC samples from the TCGA database indicated that miR-552-3p was highly expressed in HCC，and qRT-PCR showed that the expression level of miR-552-3p in HCC cell line was significantly higher than that in human liver cell line L02；the high expression of miR-552-3p promoted the proliferation，migration and invasion of HCC cells，while the inhibition of miR-552-3p reversed these functions；the luciferase reporter assays verified that miR-552-3p targets the 3′-UTR of DACH1. Up-regulation of miR-552-3p expression inhibited DACH1 whereas down-regulation of miR-552-3p increased DACH1 expression；DACH1 overexpression inhibited HCC cell proliferation，migration and invasion，but with miR-552-3p overexpression the DACH1 inhibitions of HCC cell-related functions were relieved；miR-552-3p positively regulated the activation of Wnt/β-catenin signaling pathway. Conclusion：miR-552-3p targeting DACH1 promotes HCC cell proliferation，migration and invasion，and regulates Wnt/β-catenin signaling，which may provide potential targets for HCC diagnosis and treatment strategies.