Objective：This study explores the effects and mechanisms of miR-1254 on liver cancer cells. Methods：①RT-qPCR was used to detect the expression of miR-1254 in liver cancer and adjacent tissues or cell lines. ②HUVEC tube -formation assays and transwell assays were used to demonstrate effects of miR-1254 on migration，invasion and vascular formation of liver cancer cells. ③Target Scan was used to predict the target gene of miR-1254. RT-qPCR and western blot were used to measure the expression of the target gene. Dual luciferase reporter assay was used to confirm the target gene. Results：①miR-1254 was up-regulated in liver cancer tissues and cell lines. ② miR-1254 promoted migration，invasion and vascular formation of liver cancer cells. ③The paired box gene 5（PAX5）was down-regulated in liver cancer tissues and miR-1254 negatively regulated the expression of PAX5，which was confirmed to be the direct target of miR-1254. ④Over-expressing PAX5 could reverse the promotive effects on HCC cells caused by the over-expresssion of miR-1254. Conclusion：miR-1254 promoted migration，invasion and vascular formation of liver cancer cells through targeting PAX5. So miR-1254 might have the value of target therapy in liver cancer.