Objective：This study aims to investigate the role of max action protein 1-0（Mxi1-0） in SH-SY5Y cell apoptosis induced by oxygen-glucose deprivation（OGD）. Methods：The expression levels of Mxi1-0 and mitophagy related proteins LC3-Ⅱ，p62，Tom20 in SH-SY5Y cells under OGD conditions were detected by Western blot. Mxi1-0 siRNA was tranfected into SH-SY5Y cells. The expression of Mxi1-0 and mitophagy related proteins（LC3-Ⅱ，p62，Tom20） were detected in SH-SY5Y cells under OGD using Western blot. The role of Mxi1-0 in OGD-induced apoptosis was detected using CCK-8 method and Annexin V/PI staining. Results：The expression of Mxi1-0 increased firstly and then decreased in SH-SY5Y cells after OGD treatment. The expression of mitophagy related protein LC3-Ⅱ increasesed and the expression of p62 and Tom20 proteins decreased after OGD treatment. Under OGD conditions，Mxi1-0 silence reduced mitophagy in SH-SY5Y induced by OGD，and increase the apoptosis of SH-SY5Y induced by OGD. Conclusion：Mxi1-0 antagonizes OGD induced apoptosis of SH-SY5Y cells by enhancing mitochondrial autophagy.