Objective：This study aims to evaluate the clinical value of the anthropometric waist-to-height ratio（WHtR） through the hyperinsulinemic-euglycemic clamp. Methods：A total of 154 subjects was included in the study. Lipid profiles and other biochemical markers were measured from the fasting blood sample. All the patients underwent hyperinsulinemic-euglycemic clamp，and the glucose metabolic rate，body mass index（BMI），waist circumference（WC），waist-to-hip ratio（WHR），WHtR and HOMA index were calculated. The ability of each index to predict the assessment of insulin resistance was compared. Results：Correlation analysis showed that WHtR（r=-0.730，P < 0.001），WHR（r=-0.651，P < 0.001），BMI（r=-0.717，P < 0.001），WC（r=-0.716，P < 0.001）were all significantly correlated with the glucose metabolic rate obtained in the hyperinsulinemic-euglycemic clamp. The area under ROC curve was HOMA-IR（0.860）> WHtR（0.850）> BMI（0.846）> WC（0.837）> WHt（0.749）. The best value of WHtR for the diagnosis of insulin resistance was 0.56，which showed the highest sensitivity（93.9%）and specificity（66.7%）in the ROC curve. Conclusion：WHtR has a significant correlation with the glucose metabolic rate obtained from the hyperinsulinemic-euglycemic clamp，and has a higher sensitivity and specificity than BMI，WC and WHR，suggesting that it could be widely used in epidemiological studies or some large-scale clinical trials.