Objective：To explore correlation between long chain non-coding RNA（lncRNA）BANCR and epidermal growth factor receptor-tyrosine kinase inhibitor（EGFR-TKI）drug resistance of non-small cell lung cancer. Methods：Mutated cell strain PC9 with EGFR exon 19 deletion and drug resistant cell strain PC9/GR induced by EGFR-TKI gefitinib were selected for research. Real time fluorescent quantitative PCR（qRT-PCR）was applied to examine expression difference of BANCR in PC9/GR and PC9. BANCR was over expressed in PC9/GR cells and then CCK-8 method was used to examine drug susceptibility of the cells to gefitinib and clone formation assay to examine cell reproductive capacity. Meanwhile，flow cytometry was adopted to examine cell apoptosis and Western blot to check expression of epithelial-mesenchymal transition related proteins. Results：① BANCR was significantly low expressed in PC9/GR cells（P < 0.05）. ②When BANCR was over-expressed in PC9/GR cells，compared with control group，half inhibition concentration（IC50）of gefitinib was reduced（P < 0.05），cell reproductive capacity was lowered，and cell apoptosis increased after being handled with gefitinib（P < 0.05）. ③ Compared with control group，Western blot showed that expression level of E-cadherin in the PC9/GR cells over-expressed BANCR was significantly elevated and expression level of N-cadherin was remarkably lowered（P < 0.05）. Conclusion：Long chain non-coding RNA BANCR can induce cell apoptosis and inhibit cell reproduction. Over expressed BANCR can increase sensitivity of PC9/GR cells to gefitinib and thus reverse drug resistance. This mechanism might work through regulating EMT process.