Effects and mechanism of BPS on lipid metabolism of zebrafish with high⁃fat diet
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    Abstract:

    Objective:To study the effects of bisphenol S(BPS)on lipid metabolism in zebrafish with high-fat diet and its potential mechanism. Methods:The effects of BPS in different concentration on lipid deposition in the blood vessels of zebrafish with high-fat diet were studied,and the changes of lipid metabolism-related proteins and genes were detected by ELISA and quantitative RT-PCR. Results:The cholesterol accumulation in the larvae blood vessels was significantly decreased under the exposure of 1 and 10 μg/L BPS(P < 0.01). When fed with high-fat diet 42 mg/dish,the lipid accumulation in the larvae blood vessels of the BPS exposed group was also significantly decreased(P < 0.01). The expression level of RXRα protein was significantly reduced in the 100 μg/L BPS exposed group(P < 0.05). The gene expression of hmgcrα was significantly induced by 10 μg/L BPS exposure(P < 0.05),and the mRNA levels of ldlrα and rxrα were all significantly decreased in the 100 μg/L and 1 000 μg/L BPS exposed group(all P < 0.05). While the expression of rorα gene was reduced after exposure of 0.1,1.0,100.0 μg/L BPS(all P < 0.05),and it was lower after exposure of 1 000 μg/L BPS(P < 0.01). Conclusion:The effect of BPS on lipid metabolism of zebrafish larvae was related to the feeding amount of high-fat diet and abnormal expression of lipid metabolism related genes,and its mechanism may be related to rxr/ror signaling pathway.

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余 翠,武 琳,陈 忠,杨 明.双酚S对高脂饮食斑马鱼脂代谢的影响及机制[J].南京医科大学学报(自然科学版英文版),2020,(7):981-985.

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  • Received:January 31,2020
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  • Online: August 05,2020
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