Objective：To observe the effects of 3D-printed poly lactic-co-glycolic acid（PLGA）scaffold on the repair of rat palate bone defect. Methods：The PLGA was dissolved in acetone，and disc-shaped mesh scaffolds having pores with diameters of 100 μm and a single layer thickness of 60 μm were printed using a 3D printer. Twelve rats were randomly divided into two groups（n=6）to create round palate bone defects with a diameter of 3 mm. The bone defect in the experimental group was implanted with a 3D-printed PLGA scaffold and sutured to close the defect，while in the control group the defect was sutured to close only. The rats were sacrificed after 8 weeks，and the bone defect repair was evaluated by using micro-CT and histological analysis. Results：3D-printed PLGA scaffolds were successfully fabricated. Eight weeks after the establishment of the palate defects model in rats，the bone-volume/total-volume（BV/TV）of the experimental group implanted with the 3D-printed PLGA scaffold was significantly higher than the control group（P < 0.05），and the unhealed area of the bone defect was also smaller than that of the control group（P < 0.05）. HE staining of the palate bone tissue showed that a large amount of new bone was formed at the edge of the defect in the experimental group，while the control group had only a small amount of new bone. Conclusion：3D-printed PLGA scaffold can promote the bone regeneration in palate bone defect.