Objective：To treat the rabbit model of bone tuberculosis through preparation of calcium sulphate/nano hydroxyapatite immune artificial bone，and discuss the actual effect of nanometer hydroxyapatite immune artificial bone application. Methods：Calcium sulfate/nanometer hydroxyapatite was prepared by gelatine and polyhexyl ester composite coating，the rabbit model of the right femoral immune bone tuberculosis was established by injecting tuberculosis factor. After the lesion in the bone tuberculosis model was removed，the calcium sulfate/nanometer hydroxyapatite artificial bone was plated into the defect site. The blood drug concentration of the treated group was detected at 7，14，28 days after surgery. At 4 weeks and 12 weeks after surgery，bone repair was observed under a microscope. Results：The anti-tuberculous bone of calcium sulfate/nanometer hydroxyapatite was successfully prepared，and the immune artificial bone space was basically uniform under the electron microscope，and the anti-tuberculosis drugs were dispersed between the spaces. The model of rabbit femoral tuberculosis was successfully established and the tuberculosis of bone tuberculosis was formed. In the treatment group，the blood drug concentration was maintained within 28 days after operation. Chondrocyte proliferation and the occurrence of bone healing of treatment group were active in 4 weeks after operation. At 12 weeks after surgery，the bone defect of treatment group was healed completely，and the defect was repaired by bone tissue induced by anti-tuberculous bone. The bone loss in the control group was also healed，but the bone healing was weak. Tartrate resistant acid phosphatase（TRAP）staining showed that the volume and number of osteoclasts formed decreased at 12 weeks after surgery，and the number of osteoclasts in the control group was greater than that in the treatment group. The ELISA results showed that serum C-reactive protein（CRP），interleukin-8（IL-8），interleukin-6（IL-6），tumor necrosis factor-α（TNF-α） levels decreased after treatment. Conclusion：The sulphate/nanometer hydroxyapatite immune anti-tuberculous bone can effectively treat rabbit immune bone tuberculosis，maintain the blood drug concentration for a period of time，and has a good osteogenic effect.