Objective：To investigate the distribution of stereoacuity and the relationship between stereoacuity and ocular parameters in children aged 5to 6 years. Methods：Children aged 5 to 6 years from Yuhuatai District，Nanjing City were recruited in 2017 for relevant examinations，including visual acuity（VA），ocular alignment and movements，anterior segment and fundus examination，refraction，axial length（AL），interpupillary distance，randot stereotest，etc. The distribution of stereoacuity in children aged 5 to 6 years was analyzed，and the relationship between stereoacuity and ocular parameters was analyzed by using bivariate correlation analysis and multiple linear regression model analysis. Results：A total of 1 154 children were included in the study，with stereoacuity of 40 arc seconds（652 cases）accounting for 56.5% and 60 arc seconds（388 cases）for 33.6%. Multiple linear regression showed that interocular difference in AL（P < 0.001），VA（P < 0.001）and interocular difference in spherical refractive errors（P < 0.001）were all correlated with stereoscopic vision. Conclusion：The children aged 5 to 6 years with higher interocular difference in AL，worse VA and higher interocular difference in spherical refractive errors have worse stereoacuity score.