Objective：To investigate the association between methylation of adenomatous polyposis coli（APC） gene and the risk of upper digestive tract tumor. Methods：Relevant articles were identified using PubMed，Web of Science，Embase and China National Knowledge Infrastructure（CNKI）database. We used the keywords “APC” and “methylation”，in conjunction with any of the following terms：“esophageal cancer”，“esophagus cancer”，“gastric cancer” or “stomach cancer”. References listed in the identified articles were further manually searched for additional studies. The search range was from January 2000 to February 2018. A random or fixed effect model was adopted to calculate pooled odd ratio with 95% confidence interval. Results：The current Meta-analysis included 17 studies with 1 201 cases and 959 controls. The significant heterogeneity was across studies. The pooled OR of APC methylation was 13.24（95% CI：6.42-27.33，P < 0.001）for upper digestive tract tumor. A significant association was found between the hypermethylation frequency and the increased TNM stages（pooled OR：3.95，95% CI：1.46-10.66，P=0.007）. Conclusion：The present Meta-analysis provides evidence that APC promoter methylation is associated with an increased risk in upper digestive tract tumor. Our findings underscore the clinical relevance of aberrant methylation as a promising biomarker for upper digestive tract tumor.