Roles of small RNA 2’⁃O⁃methyltransferase Henmt1 during mouse spermatogenesis
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    Abstract:

    Objective:This study aims to observe the role of small RNA 2’-O-methyltransferase Henmt1 during mouse spermatogenesis by generation of Henmt1 mutant mouse model. Methods:Henmt1 mutant mouse were constructed by CRISPR/Cas9 system(clustered regularly interspaced short palindromic repeats and CRISPR-associated protein 9)and analyzed the phenotype of mutant mice by computer-aided sperm analysis,HE staining and immunofluorescent staining. Results:Henmt1 mutant mouse model were successfully generated. Henmt1 mutant males are sterility with spermatogenic arrest and up-regulation of retrotransposon LINE1. Conclusion:This study found that HENMT1 plays an important regulatory role during spermatogenesis.

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戴馨缘,王仿竹,靳佳川,李 伟,沈 彬.小RNA 2’⁃O⁃甲基转移酶Henmt1在小鼠精子发生中的作用[J].南京医科大学学报(自然科学版英文版),2020,(9):1263-1268.

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  • Received:June 28,2020
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  • Online: September 30,2020
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