Objective：To investigate the role of Gef26 in Drosophilia primary culture. Methods：Through primary culture of Drosophilia wide type，gef26 mutants and gef266/+；Abl4/+ mutants，the cell shape is observed，and by fluorescent staining of F-actin the distribution of cytoskeleton can be observed. Results：In Drosophila primary culture, wild type muscle cells have normal morphology, and the morphology of muscle cells of the gef26 mutants is obviously abnormal. The gef266/+;Abl4/+ mutants muscle cells have a normal morphology, namely the phenotype of gef26 mutant muscle cells with abnormal morphology rescued by Abelson tyrosine kinase（Abl） mutation. F-actin in wild type muscle cells showed a regular morphology and continuous arrangement, while that in the gef26 mutants muscle cells showed irregular morphology and discontinuous arrangement. Meanwhile, F-actin in gef266/+; Abl4/+ double mutant cells showed a regular morphology and continuous arrangement, that is, the phenotype of gef26 mutant with disordered F-actin arrangement rescued by Abl mutation. Conclusion：Gef26 regulates cell cytoskeleton organization through Abl signaling during muscle cells development.