Bone tissue engineering study of DMOG⁃loaded photocrosslinked GelMA hydrogel
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    Abstract:

    Objective:To investigate the effect of dimethyloxallyl glycine(DMOG)-containing gelatin methacryloyl(GelMA)hydrogel on promoting osteogenic differentiation in vitro,and the effect of repairing calvarial bone defects in rats in vivo. Methods:After synthesizing GelMA-DMOG hydrogel,the surface characteristics were observed by scanning electron microscope,and its degradation time was detected in vitro. Mouse osteoblast precursor cells MC3T3-E1 were cultured on the surface of GelMA and GelMA-DMOG hydrogel. The cytoskeleton morphology was observed by fluorescence,and its cytotoxicity was detected by CCK-8 assay. After cultured cells in osteogenesis induction medium,alkaline phosphatase(ALP),alizarin red staining and qRT-PCR were used to detect the effect of two kinds of hydrogel on osteogenic differentiation of MC3T3-E1 cells. A rat calvarial bone defect model was constructed. The effects of GelMA and GelMA-DMOG hydrogels on bone defect repair were observed after 4 and 8 weeks. Results:Compared with GelMA hydrogel,there was no significant difference in the pore size,distribution,and degradation time of GelMA-DMOG hydrogel. The two groups of hydrogel had no significant effect on the proliferation of MC3T3-E1 cells. Compared with control group,the GelMA hydrogel cultured group slightly promoted osteogenic differentiation of MC3T3-E1 cells,while the GelMA-DMOG hydrogel cultured group significantly enhanced the osteogenic differentiation and the expression of osteogenesis-related genes,such as Runx2,ALP,and osteocalcin(OCN). In vivo,compared with control and GelMA group,the GelMA-DMOG hydrogel group has a much better calvarial bone repair effect. Conclusion:The physical and chemical properties of GelMA-DMOG hydrogel are not changed compared to GelMA hydrogel. In vitro and in vivo experiments show that the GelMA-DMOG hydrogel can promote the repair of bone defects,which opens up a new clinical application of bone tissue engineering in the treatment of bone defects.

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张燕萍,陈花花,顾永春,周培刚.负载DMOG光交联明胶水凝胶的骨组织工程研究[J].南京医科大学学报(自然科学版英文版),2020,(10):1449-1455.

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  • Received:January 25,2020
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  • Online: November 10,2020
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