The first monoclonal antibody was approved by the Food and Drug Administration（FDA） in 1986. Over the past 30 years，the antibody field has developed rapidly. By December 2019，79 monoclonal antibodies have been approved by FDA for the treatment of cancer，autoimmune，infectious and other diseases. Antibodies have the advantages of high specificity and less side effects，and have become the mainstream of new drug development. Among the top ten biological drugs in the world，antibodies account for half of the total. This paper introduces the novel research technologies，including antibody humanization，phage display，transgenic mice，and single cell PCR，etc.