Objective：This study aims to investigate the correlation between the metabolic parameters of 18F-FDG PET/CT and the expression of neuroendocrine markers（Syn，CgA，CD56），TTF-1 and Ki-67，and the value of prognosis in small cell lung cancer（SCLC）. Methods：The immunohistochemical data and 18F-FDG PET/CT data of 96 SCLC cases confirmed by pathology were retrospectively analyzed，the cases collected from January 2009 to May 2017 in the First Affiliated Hospital of Nanjing Medical University. Results：The median of primary lesion maximum standardized uptake value（PSUVmax），whole body lesion metabolic tumor volume（WBMTV）and whole body lesion glycolysis（WBTLG）in 96 SCLC patients were 10.30（1.20~42.90）、108.44（0.41~710.40）cm3、585.73（1.30~10 668.50）；the values of PSUVmax，WBMTV and WBTLG had statistically significant differences between the negative and positive groups of TTF-1（Z=-3.063，-2.235，-2.538，P=0.002，0.025，0.011）. The Spearman correlation analysis shows that there was a positive correlation between PSUVmax and Ki-67 expression（r=0.620，P < 0.001）. Kaplan-Meier single factor analysis shows the expression of TTF-1 and CD56，WBMTV，WBTLG was significantly correlated with overall survival（HR：0.477，0.090，2.862，2.795，all P < 0.05）. Multivariate survival analysis showed that CD56 and WBMTV were independent prognostic factors of overall survival（OS）. Conclusion：The metabolic parameters of 18F-FDG PET/CT in SCLC had a good correlation with the expressions of TTF-1 and Ki-67，and no correlation with neuroendocrine markers. CD56 and WBMTV were the independent prognostic factors of OS.