Objective：This study aims to explore the molecular mechanism of matrine in inhibiting the invasion and migration of human cervical cancer cell line Caski cells based on the Wnt/β-catenin pathway. Methods：Caski cells were treated with different concentration of matrine. Methyl thiazolyl tetrazolium（MTT）assay was used to detect cell proliferation inhibition；Transwell chamber invasion and migration assay was used to detect cell invasion and migration；ELISA assay was used to detect cell matrix metalloprotein 9（MMP-9）and vascular endothelial growth factor（VEGF）secretion；Quantitative real-time PCR（qRT-PCR）was used to detect cell glycogen synthase kinase3β（GSK-3β）and wingless/integrated 2B（Wnt2B）expression；Western blot was used to detect cell β-catenin，p-β-catenin protein content. Results：Matrine had inhibitory effect on the proliferation of cervical cancer cells Caski cells（P < 0.05）and showed a time and concentration dependence. Matrine inhibited the invasion and migration of Caski cells（P < 0.05），and it had a significant effect on Caski cells. The ability to secrete MMP-9 and VEGF has a significant inhibitory effect（P < 0.05）；matrine can significantly reduce Wnt2B mRNA expression and β-catenin protein content in Caski cells，and increase GSK-3β mRNA expression in Caski cells and p-β-catenin protein content. Conclusion：Matrine promotes the expression of GSK-3βmRNA in Wnt/β-catenin pathway of Caski cells，increases the content of p-β-catenin protein，reduces the expression of Wnt2B mRNA，the content of β-catenin protein and the secretion of MMP-9 and VEGF in Caski cells，thereby inhibiting the invasion and migration of Caski cells.