Objective：To establish a fluorescence immunochromatography for placental growth factor（PlGF），evaluate the analysis performance and clinical performance. Methods：Using fluorescent microsphere conjugated-mouse-anti-human PlGF monoclonal antibody，sheep anti-human PlGF polyclonal antibody coated on NC membrane，to establish a fluorescence immunochromatography assay for PlGF，and evaluate the analysis performance，such as linear range，accuracy，repeatability. Using serums of 136 pregnant women，electrochemiluminescence as control，to evaluate the clinical performance. Results：The linear range of fluorescence immunochromatography assay for PlGF was 20.0~9 000.0 pg/mL，accurate was 97.98%，repeatability CV was 3.14%（500.0 pg/mL）and 2.98%（5 000.0 pg/mL）. The assay had good correlation with electrochemiluminescence：y=1.032x-5.002（r=0.991）. Bland-Altman statistical analysis showed that the average value of fluorescence immunochromatography was slightly higher than that of electrochemiluminescence，without significant difference（P > 0.05）. Conclusion：The fluorescence immunochromatography for PlGF has good analysis performance and clinical performance. The assay is suitable for clinical application.