Objective：This study aims to explore influences of recombinant chromodomain helicase DNA binding protein 4（CHD4）gene expression on proliferation and apoptosis of acute T lymphoblastic leukemia cells and to identify its promoter. Methods：siRNA-CHD4 was used to transiently transfect Jurkat cells to knock down the expression of CHD4. The qRT-PCR and Western blot were used to detect the expression of CHD4. The apoptosis rate and cell cycle were measured by flow cytometry. The effect of CHD4 gene on the proliferation of Jurkat cells was analyzed by CCK-8. According to bioinformatics analysis，a 2 091 bp fragment of CHD4 gene candidate promoter region was amplified by PCR using the whole genome DNA extracted from Jurkat cells as template. The sequence containing candidate promoter region of CHD4 gene was cloned with pGL3 basic as vector，and a series of plasmids containing truncated 5′ flanking region of CHD4 gene candidate promoter were constructed. The plasmids containing CHD4 promoter and truncated sequence were transfected into Jurkat and HEK293T cells. The promoter activity of each fragment was detected by double luciferase reporter gene，and the minimal active region of CHD4 gene promoter was determined. The effect of binding site on the transcription of CHD4 was analyzed by double luciferase reporter gene detection. Results：Flow cytometry showed that，compared with the control group，CHD4 inhibited the apoptosis of Jurkat cells，the Jurkat cells transfected siRNA-CHD4 were significantly increased in the G0/G1 phase and decreased in the S phase（P < 0.01）. CCK-8 essay identified that CHD4 gene promoted the proliferation of Jurkat cells（P < 0.05）. Plasmids containing CHD4 gene candidate promoter and truncated sequence plasmids were successfully constructed. Compared with empty vector，plasmids containing CHD4 gene candidate promoter sequences were significantly more active（P < 0.05）. The core promoter of CHD4 gene located in 233 bp to 13 bp relative to transcription start site，which contained transcription factor binding sites NF-κB and MZF1. NF-κB had a positive function to the CHD4 promoter activity. Conclusion：Our results suggested that CHD4 inhibited apoptosis and induced proliferation in Jurkat cells. The core promoter of CHD4 located in -233/-13 bp relative to TSS. NF-κB bound to the core promoter of CHD4 in vivo and it had positive function to the promoter activity of CHD4.