Objective：This study aims to systematically compare the methods for detecting the proliferation and cytotoxic function of immune effector cells. Methods：CCK- 8 assay，EdU labeling assay and CFSE labeling assay were used to detect the proliferation of NK92 cells in vitro under different culture conditions（group 1：100 U/mL IL-2；group 2：100 U/mL IL-2+10 U/mL IL-15），and the killing effect of NK92 on K562 cells was detected by lactate dehydrogenase（LDH）release assay，double living cell dye labeling assay and luciferase assay. The principles，characteristics and effectiveness of the various methods were compared. Results：The results of CCK-8 assay showed that the proliferation ability of group 2 was stronger than that of group 1，but there was no significant difference between group 2 and group 1（P > 0.05），but both EdU and CFSE labeling assay showed that there were significant differences in proliferation ability between the two groups. Double living cell dye labeling flow assay and luciferase assay could detect the differences in cytotoxic function of NK92 between the two groups under different effector-target ratio（P < 0.05），but there was no difference in cytotoxicity between the two groups under low effector-target ratio detected by LDH release assay（P > 0.05）. When double living cell dye labeling flow assay was as standard method，results of luciferase assay and LDH release assay were significantly correlated with the results of living cell dye labeling flow assay（rS < 0.979 4，P < 0.001；rS=0.973 2，P < 0.001）. Conclusion：CCK-8 assay is more focused on the detection of metabolic activity，EdU and CFSE labeling assay are more suitable for detecting the proliferation of suspended immune cells. The three kinds of cytotoxic function assay are consistent. Double living cell dye labeling assay is suitable for detecting the killing of suspended target cells，and luciferase assay is more suitable than LDH release method for the killing of adherent cells.