Objective：To investigate the change of HIV subtypes and drug resistance in the follow-up HIV/AIDS population in Wuxi，Jiangsu in 2014 and 2016. Methods：HIV molecular epidemiological survey and follow-up study were carried out among HIV/AIDS patients who did not receive antiviral treatment in Wuxi in 2014. The basic information of the infected person was collected and DNA was extracted from blood samples. The pol，env，and gag gene fragments of HIV were amplified by nest-PCR and sequenced to determine the HIV genotype of the infected person. The pol sequence was submitted to the HIV drug resistance database for drug resistance mutation site analysis. In 2016，the same population was followed up and selected for antiviral treatment. HIV subtypes and drug resistance mutations were detected again，and the changes of subtypes and drug resistance in two years were analyzed. Results：A total of 117 HIV/AIDS cases were collected，and 234 samples were detected. Seven subtypes of HIV-1，CRF01_ AE，CRF07_ BC，B，CRF67_ 01B，CRF08_ BC，CRF55_ 01B，and CRF68_ 01B，and six unique recombinant types（URFs）were detected. The transmitted drug resistance rate was 4.95% in 2014，acquired drug resistance rate was 5.75% in 2016. The drug resistance of 5 cases changed，and one of the subtypes changed. Conclusion：The HIV-1 subtypes of HIV/AIDS patients in Wuxi are diverse and complex. The subtypes and drug resistance have changed greatly. Therefore，it is necessary to strengthen the surveillance of subtypes and drug resistance mutations.