Objective：This study aims to investigate the effects of bovine serum albumin（BSA）coated Ag2S storage solution on the surface characteristics of pure titanium and the behaviors of osteoblasts. Methods：After polishing and cleaning，pure titanium disks were stored in air，saline，BSA solution and BSA-coated Ag2S storage solution（Ag2S@BSA） respectively for two weeks. Scanning electron microscope（SEM），energy dispersive spectrometer（EDS） and contact angle test were used to analyze surface characteristics of pure titanium preserved in four media. Assays of adhesion，proliferation，and differentiation of MC3T3-E1 cells were conducted to evaluate the biocompatibility of different samples. Results：SEM and EDS results demonstrated that sodium chloride crystals were scattered on titanium surface in the saline group，while carbon and oxygen distribution were denser in the BSA group. Nano-sized particles containing silver and sulfur were adsorbed on the titanium surface without changing the original morphology. Compared with the other three groups，titanium surfaces preserved in Ag2S@BSA solution showed superior hydrophilicity and significantly promoted the adhesion proliferation and differentiation abilities of osteoblasts with high expression of osteogenesis-related proteins. Conclusion：The Ag2S@ BSA-containing storage solution can enhance the osteogenic activity of osteoblasts by improving the surface characteristics of pure titanium.