Objective：This study aims to observe the effects and mechanisms of Wnt5a on the formation of neutrophil extracellular trap（NET）. Methods：Neutrophils were stimulated with 50 ng/mL Wnt5a for 3 h，1 μg/mL Porphyromonas gingivalis （P.gingivalis）lipopolysaccharide（LPS） and 1 μg/mL Escherichia coli（E.coli） LPS were served as positive controls. Formation of NET was observed by immunofluorescence staining，and extracellular DNA was quantified by Sytox Green fluorescence staining. Levels of reactive oxygen species（ROS） were detected by flow cytometry，and activations of phosphatidylinositol 3-kinase（PI3K）/Akt，mitogen-activated protein kinase 1/2（MEK1/2）and extracellular regulated protein kinases 1/2（ERK1/2） were explored by Western blot. Results：Compared with neutrophils without stimulations，Wnt5a stimulation led to the formation of NET and the increased levels of extracellular DNA（P < 0.05）. In addition，levels of ROS and phosphorylations of PI3K/Akt，MEK1/2 and ERK1/2 were significantly increased in the cells treated with Wnt5a（P < 0.05）. Conclusion：Wnt5a might contribute to the formation of NET depending on the production of ROS and the phosphorylations of PI3K/Akt，MEK1/2 and ERK1/2.