Objective：This study aims to use bioinformatics methods to screen the differentially long non-coding RNA（LncRNA） in vaginal epithelial tissues of women with vaginal lubrication disorder（LD），and to construct patients with vaginal lubrication disorder based on the hypothesis of lncRNA-mRNA co-expression network to further explore the potential pathogenesis of patients with vaginal LD，in order to provide new ideas for the diagnosis and treatment of patients with vaginal LD. Methods：Using |Log2FC|≥2 and correct P ralue＜0.05，the expression profile of long-chain non-coding RNA and mRNA between LD group and normal control group were identified. Using Cytoscape software，a lncRNA-mRNA network of candidate lncRNAs was constructed. Using Gene Ontology（GO）and KEGG Pathway，the biological functions of mRNAs in co-expression network were analyzed. Results：A total of 499 up-regulated lncRNAs，337 down-regulated lncRNAs，and 1 582 up-regulated mRNAs，633 were selected in the LD group and the normal control group based on the next-generation sequencing technology according to the criteria of |Log2FC|≥2 and correct P ralue＜0.05. Subsequently，a lncRNA-mRNA co-expression network was constructed based on differentially expressed lncRNA and mRNA through 100 lncRNAs and 311 mRNAs. Finally，the functional enrichment analysis of the lncRNA-mRNA network showed that mRNA was mainly related to myocardial-related diseases and functions. Conclusion：The pathogenesis of LD may be related to blood circulation dysfunction，local muscle dysfunction，and cGMP pathway，etc. This requires further studies at the cellular level，animal level and even human level to confirm.