Recent studies have found that long non-coding RNA（LncRNA）affect the occurrence and development of tumors by regulating genes related to cell proliferation and apoptosis. LncRNA ZEB2-AS1 regulates various aspects of tumor cell biological behaviors including proliferation，migration，invasion，apoptosis and glycolysis，both in vivo and in vitro. This review summarizes the research progress of the effect of ZEB2-AS1 on tumor biological behaviors and its working mechanisms，and evaluates whether it can be considered as a therapeutic target and biomarker for tumor diagnosis and prognosis.