Objective：This study aims to explore the role and potential molecular mechanism of microRNA（miR）-190 in aging-related energy metabolism disorders. Methods：Real-time fluorescent quantitative PCR（RT-PCR）was used to detect the expression level of miR-190 in the adipose tissue of aging mice；a cellular senescence model was constructed and RT-PCR was used to detect the changes of miR-190 in adipocytes；RT-PCR was used to detect the changes in thermogenesis genes in the adipocytes transfected with miR-190；the target genes of miR-190 were verified by the dual luciferase reporter gene experiment；the metabolic homeostasis was determined in aged mice injected with miR-190 antagomir. Results：During the aging process，the expression of miR-190 in adipose tissue was elevated；in the senescent cell model，the expression of miR-190 was also up-regulated；overexpression of miR-190 in adipocytes would lead to a decrease in the expression of thermogenesis genes；the key genes for thermogenesis PRDM16 is the direct target gene of miR-190；inhibiting the level of miR-190 in aging mice can help improve the energy metabolism homeostasis of aged mice. Conclusion：miR-190 may affect adipose tissue heat production by targeting PRDM16，which in turn affects energy metabolism homeostasis and accelerates aging.