Objective：To analyze the differences of lncRNA in cultured bone marrow mesenchymal stem cells（BMSCs） in osteoporotic model rats and the functions of the lncRNA. Methods：Twelve SD rats were equally divided into the osteoporosis model group and the control group. The osteoporosis model was made by ovariectomy. Bone mineral density（BMD） in ovariectomized rats and sham control were determined by dual-energy X-ray bone mineral density detector. BMSCs from femur bone marrow were cultured in vitro. The different expression of lncRNA in BMSCs of osteoporosis model（n=3） and control group（n=3） was detected by gene chip technology, and the different biological functions of lncRNA and related signaling pathway were analyzed by GO function analysis and the KEGG enrichment. Results：BMD in osteoporosis model rats［（157.33±6.28）mg/cm2］，was lower than the control group rats［（183.33±4.50）mg/cm2］ significantly（P < 0.05）. A total of 32 759 gene fragments were detected in BMSCs in the osteoporosis model group and the control group, and 8 454 lncRNA gene fragments had significant difference（P < 0.05），among which 3 593 were up-regulated and 4 861 were down-regulated in osteoporosis model group. Totally 4 992 lncRNA gene fragments were detected，and there were significant differences in 116 lncRNA gene fragments in BMSCs of osteoporosis model rats，with 35 increased and 81 decreased. Differential lncRNAs are mainly involved in the biological process of cell growth and development，and regulate protein transport pathways. Conclusion：There were differences in the expression of lncRNA in BMSCs between osteoporosis model rats and normal rats, and the differentially expressed lncRNA involved in the regulation of BMSCs growth and development process or protein transport pathway.