Objective：We investigated whether genetic variants of HNF1A and HNF1B were associated with the risk of breast cancer in a Chinese Han population. Methods：A case-control study of 1 032 breast cancer cases and 1 063 cancer-free female controls was performed，and six single nucleotide polymorphisms（SNPs）in HNF1A and HNF1B were genotyped in Illumina genotyping platform. Logistic regression was used to calculate the odds ratios（ORs）and 95% confidence intervals（CIs）for the association between a single locus and breast cancer risk with adjustment for age，age at menarche，and menopausal status. Results：Multiple logistic regression analysis illustrated that three SNPs within HNF1A gene were associated with significantly decreased risk of breast cancer（rs2464196，OR=0.80，95%CI：0.71~0.91，P=7.45×10-4；rs1183910，OR=0.87，95%CI：0.77~0.99，P=0.039；rs7310409，OR=0.86，95% CI：0.76~0.98，P=0.023）. After correction for multiple comparisons（FDR<0.05），rs2464196 was still significantly associated with susceptibility to breast cancer（P=4.47×10-3）. Conclusion：The SNP rs2464196 within HNF1A gene may be a candidate marker for susceptibility to breast cancer in Chinese Han population.