Objective：This study aims to investigate the role of miR-484 in adipogenesis and its clinical potentiality. Methods：To explore the function of miR-484 in human pre-adipocyte（HPA）differentiation and proliferation，RT-qPCR，CCK-8，red oil staining and triglyceride content assays were conducted. Additionally，a dual-luciferase reporter assay demonstrated MAPK8，which predicted by Targetscan7.2，was a direct target gene of miR-484 during preadipocyte differentiation，and the results were confirmed by RT-qPCR and Western blot. Results：The miR-484 was down-regulated during HPA differentiation. The miR-484 inhibited the proliferation and differentiation in HPA，and silencing miR-484 had the opposite effects. A dual-luciferase reporter assay demonstrated MAPK8 was a direct target gene of miR-484. Additionally，over expression of MAPK8 could reserve the negative effect of miR-484 on differentiation and proliferation of pre-adipocytes. Conclusion：MiR-484 could inhibit HPA differentiation and proliferation by targeting MAPK8.