Objective: To realize the status of university students’ health literacy in Nanjing city and analyze the correlative influence factors. Methods: Based on the questionnaire survey of Chinese citizens’ health literacy in 2008, a questionnaire survey of university students’ health literacy was designed, and conducted among 615 university students drawn from freshman to senior by using the multistage cluster sampling method. Results: 1.8% university students had health literacy; the rates of heath knowledge and concept, health life style and behavior and health skills were 15.4%, 0.7% and 36.7% respectively. The influence factors of university students’ health literacy were correlative to gender, place of graduation, parents’ level of education, grades and academic record. Conclusion: The level of university students’ health literacy is lower. The university educators should focus on the survey and study of health literacy of university students, formulate a unified standards about policy of health education and promotion, directly develop health literacy education, and increase the level of students’ health literacy.