Objective: To study subjective well-being and personal affecting factors among clinical college students. Methods: General well-being schedule(GWB), Beck depression inventory(BDI), Eysenck personality questionnaire(EPQ-RSC), and the self-esteem scale(SES) were used. Results: The clinical college students had a high level of subjective well-being. Extroversion was positively related to subjective well-being, and neuroticism was negatively related to subjective well-being. The self-esteem was negatively related to subjective well-being. The total score of depression and its items were negatively related to subjective well-being. Neuroticism was the most powerful and stable factor predicting subjective well-being; the depressed attitude, physical symptoms and depressed actions were also second powerful factors predicting subjective well-being, among which, feeling of exhaustion and sad mood were comparatively effective; while the self-esteem only explain a small part of subjective well-being. Conclusion: Some suggestions were proposed according to these personal affecting factors in order to enhance the subjective well-being and mental health of the medical students.